The flow cytometers Diaries
The flow cytometers Diaries
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Information-enriched characterization of mobile populations. Morphological data from photos can insert into the richness of flow cytometry details. By way of example, the figure displays an normally common apoptosis assay using Annexin V and PI, incorporating mobile imaging to characterize cells in each inhabitants to reveal morphologically unique attributes. These insights couldn't have already been gained from multiplex staining by itself.
As samples are acquired on the Attune CytPix Flow Cytometer, the high-velocity brightfield digicam captures and shops illustrations or photos of detected functions in a amount around 6,000 visuals for each next, depending on the flow fee and impression dimension. For greater overall flexibility, Attune Cytometric Computer software permits you to alter the impression seize frequency as desired and choose a selected gate for image seize, if essential.
Merge acoustic concentrating engineering with high-parameter Examination for versatile spectral unmixing and traditional workflows.
Discover new insights into your experimental details by visualizing populations making use of blended fluorescence and morphology information. Our computer software interface wizards allow downsampling, concatenation (merged files) and dimensionality resources which have been designed to the application for an intuitive, streamlined info Examination practical experience.
"A picture is not rather well worth one thousand dots, but it surely really helps to see visuals. It is great to see images that correlate with staining. For instance, AnnexinV-positive cells which might be morphologically unique than healthier cells, or CD14+ monocytes that are greater and a lot more textured than CD3+/CD4+/CD14- lymphocytes.
In addition, it really is illuminating to discover how many doublets slip as a result of common singlet gates, and fantastic to verify the status of unforeseen double-beneficial events in my Evaluation."
Optimizing gating tactic. Even strong manual singlet gating is mistake-inclined and stays a subjective determination stage in Practically all flow cytometry assays. Imaging can be employed to substantiate and change gates to include only one cells of interest.
Constant graphic quality even at significant throughput Acoustic concentrating in addition to a higher-pace digicam Incorporate to picture these T cells consistently at very low or substantial flow fees. Conveniently regulate concentrate and camera settings to satisfy experimental prerequisites.
The Attune NxT cytometer is the reputable workhorse instrument your lab can depend upon for reliable and dependable effectiveness. The Attune CytPix design features a large-pace brightfield camera and AI-driven picture Investigation application, with current upgrades which includes equipment Discovering resources and an intuitive consumer interface, enabling Improved info precision, precision, and more quickly insights into sample biology.
Consumers can check out function pictures in serious-time to verify sample quality, enhance protocols, and report the morphology of each cell population identified for Evaluation. Imaging capture also Positive aspects from acoustic focusing, which really helps to position the cells so that a sharp, centered graphic is attained.
AI-driven automated graphic Examination Together with the Attune Cytometric Software interprets event options into distinct morphology parameters that can be coupled with standard fluorescence and scatter parameters to reveal highly reproducible population variations and distinctive subsets.
Application note: Automated image analysis reduces consumer-to-user variability in flow cytometry gating procedures
Leverage the power of advanced technological innovation and innovation to speed up your discoveries. Have confidence in while in the Attune Flow Cytometer family members to deliver the dependability, speed, and accuracy that set your do the job apart.
Adaptable payment plan from Agilent Monetary Answers enable you to receive the vital instrumentation you may need for fast task, or to grow your lab.
At the guts of each and every Attune Flow Cytometer lies our revolutionary acoustic concentrating technologies, which utilizes audio waves to meticulously align cells inside the flow mobile. This innovative approach surpasses common hydrodynamic approaches, causing a lot quicker processing speeds, Excellent facts high quality, and increased sample throughput.
As the cell line had previously been analyzed over the Attune CytPix Flow Cytometer, the scientists went back again to the photographs and had been capable of click here document the microbial infection a minimum of 5 times earlier.
The Attune CytPix Flow Cytometer makes it possible for buyers to visualize samples for the duration of acquisition, validate the accuracy of gating strategies, and support effects with dependable documentation for publication reasons.
What seemed to be a homogeneous populace is really more diverse—an insight that should be considered when interpreting outcomes.