A Simple Key For cytometer Unveiled
A Simple Key For cytometer Unveiled
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Info-enriched characterization of cell populations. Morphological information and facts from photographs can include towards the richness of flow cytometry details. For instance, the figure shows an if not typical apoptosis assay working with Annexin V and PI, introducing cell imaging to characterize cells in Every single inhabitants to expose morphologically distinctive options. These insights could not happen to be attained from multiplex staining on your own.
Whether you’re a starter or a professional, check out our sources to advance your research and abilities in flow cytometry.
(B) RAW 264.7 macrophages are sometimes annotated inaccurately with regard to particle count due to their complexity and noncircular borders. The quantity of singlets readily available for Investigation will increase once the person trains and employs a far more correct design.
Learn new insights into your experimental details by visualizing populations using combined fluorescence and morphology info. Our software program interface wizards empower downsampling, concatenation (merged documents) and dimensionality resources which have been developed into the computer software for an intuitive, streamlined knowledge Examination experience.
"A picture is just not quite well worth a thousand dots, but it definitely helps to see images. It is excellent to see illustrations or photos that correlate with staining. For example, AnnexinV-favourable cells which can be morphologically distinctive than healthy cells, or CD14+ monocytes that are bigger and a lot more textured than CD3+/CD4+/CD14- lymphocytes.
On top of that, it is illuminating to view the number of doublets slip as a result of classic singlet gates, and terrific to confirm the status of unanticipated double-good situations in my Assessment."
Pair your Attune flow cytometer with an autosampler for elevated workflow effectiveness, and insert robotic integration for max throughput.
Regular graphic top quality even at significant throughput Acoustic focusing and a significant-speed digicam Merge to impression these T cells continuously at low or substantial flow prices. Simply modify concentration and camera configurations to satisfy experimental demands.
Early indications of this contamination had at first been dismissed as particles. Backgating to find new populations. Backgating imaged cells over the Attune CytPix Flow Cytometer also permits you to use morphological features to discover attention-grabbing subpopulations that might not be apparent from flow cytometry facts by yourself.
Perhaps most importantly, these functions include cells of Evidently diverse phenotypes which could lead on to incorrect conclusions about double favourable situations (particularly in unusual populations).
AI-driven automatic impression Evaluation Together with the Attune Cytometric Software translates function characteristics into unique morphology parameters that may be combined with normal fluorescence and scatter parameters to expose extremely reproducible populace changes and exceptional subsets.
We Formerly demonstrated the strength of imaging Auto-T/Ramos mobile interactions. Permit’s check out just the population of finest interest, the double favourable activities, To find out more. We could now use prolonged morphological parameters (listed here, circularity and skewness of depth) to even further study the characteristics of these populations and refine gating on these functions, increasing info robustness.
Developed-in dimensionality reduction resources enhanced resolution into populations. Fluorescently gated populations back again-gated on to UMAP plot to confirm anticipated inhabitants resolution in lysed entire human blood. Consumer can view occasions from each cluster for visual confirmation of cell phenotypes.
Flexible payment program from Agilent Money Remedies let you get the crucial instrumentation you'll need for instant job, or to expand your lab.
At the center of each Attune Flow Cytometer lies our groundbreaking acoustic concentrating technological innovation, which makes use of sound waves to meticulously align cells inside the flow cell. This progressive solution surpasses regular hydrodynamic approaches, resulting in faster processing speeds, Remarkable info good quality, and increased sample throughput.
Because the mobile line had previously been analyzed about the Attune CytPix Flow Cytometer, the researchers went back again to the pictures and have been ready to doc the microbial an infection at the least five times previously.
Agilent Benefit Assure guarantees you at the least ten years of instrument use with the day you purchase. Usually, We are going to credit rating you with website the residual value of the procedure towards an upgraded model.
What appeared to be a homogeneous inhabitants is definitely extra assorted—an insight that should be thought of when interpreting effects.